Litcius/Paper detail

Microwell array chip-based single-cell analysis

Jin Zhang, Jing Xue, Ningfeng Luo, Feng Chen, Badong Chen, Yongxi Zhao

2023Lab on a Chip33 citationsDOIOpen Access PDF

Abstract

analysis ability, making it an ideal technique for single-cell studies. Herein, recent advances of single-cell analysis based on microwell array chips are summarized. We first introduce the design and preparation of different microwell chips. Then microwell-based cell capture and lysis strategies are discussed. We finally focus on advanced microwell-based analysis of single-cell proteins, nucleic acids, and metabolites. The challenges and opportunities for the development of microwell-based single-cell analysis are also presented.

Topics & Concepts

MicrofluidicsSingle-cell analysisComputer scienceNanotechnologyProfiling (computer programming)Computational biologyCellChemistryBiologyMaterials scienceOperating systemBiochemistryInnovative Microfluidic and Catalytic Techniques InnovationMicrofluidic and Bio-sensing TechnologiesMicrofluidic and Capillary Electrophoresis Applications