Synthesis and Characterization of Gum Kondagogu Stabilized Zinc Oxide Nanoparticles and Its Application as an Antibacterial Agent Against Wastewaterborne Bacteria
Aruna Jyothi Kora, Venkata Balarama Krishna Mullapudi
Abstract
Wastewater recycling is one of the viable options for attaining sustainable water management. In this scenario, gum stabilized zinc oxide nanoparticles (ZnO NP) were synthesized employing the alkaline precipitation method with gum kondagogu stabilizer. Synthesized NP were characterized using UV-vis, DLS, zeta potential, XRD, FTIR, and TEM. ZnO NP exhibited an absorption peak at 351 nm in UV-vis, a zeta potential value of-35 mV, and a z-average value of 254 nm in DLS. XRD pattern showed a characteristic hexagonal wurtzite crystal structure and FTIR data indicated the capping of NP by various gum functional groups. The size of spherical NP varied from 12.4 to 35.7 nm, and the mean particle size was 24.8 ± 5.1 nm. The in vitro antibacterial activity of ZnO NP against wastewaterborne bacteria, Escherichia coli (Gram- negative) and Bacillus subtilis (Gram-positive) was studied with the resazurin broth assay. MIC values of 900 μg/mL and 900 μg/mL and MBC values of 1800 μg/mL and 900 μg/mL of ZnO NP were recorded towards E. coli and B. subtilis. Notably, ZnO NP exhibited superior bactericidal activity against B. subtilis compared to previous studies. Thus, the current study on ZnO NP mediated bacterial disinfection has wide applications in wastewater treatment and remediation of microbially contaminated effluents.