Detection of clenbuterol in meat samples using a molecularly imprinted electrochemical sensor with MnFe2O4-CQDs composite material
Chong Lin, Yuran Li
Abstract
Clenbuterol hydrochloride (CLB) is a β-adrenergic agonizts that can stimulate specific receptors in muscles, leading to an increase in protein synthesis and lipid solubility, and consequently muscle fiber growth. As a result, it is a prohibited substance in sports. In this study, an electrochemical sensor based on molecular imprinting technology was proposed for high-sensitivity detection of CLB. MnFe2O4-carbon quantum dots (CQDs) composite material was prepared by a one-step solvent-thermal method and used as the electrode modification material to prepare the modified electrode. MIP (molecularly imprinted polymer) film was electro-polymerized using 3-aminophenylboronic acid as the functional monomer and CLB as the template molecule with the CV (cyclic voltammetry) technique. After washing out the CLB template molecule with an acetic acid-sodium acetate buffer solution, an MIP/MnFe2O4-CQDs-CS/GCE sensor with specific recognition performance for CLB was obtained. The factors that affect the performance of the sensor were investigated, including sensitivity, selectivity, stability, reproducibility, and repeatability. The research results showed that under the optimal conditions, the constructed sensor could detect CLB in the concentration range of 10 nM to 10 mM, with a calculated LOD of 0.041 μM.