Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis
Chandra Sugiarto Wijaya, Xinan Meng, Qingxian Yang, Suhong Xu
Abstract
Efficient membrane repair after injury is essential for cell and animal survival. Caenorhabditis elegans epidermal cell hpy7 has emerged as a powerful genetic system to investigate the molecular mechanism of membrane repair in vivo. This protocol describes detailed approaches for how to perform wounding on the epidermis and how to examine membrane repair by trypan blue staining, confocal imaging, and data analysis. For details on the use and execution of this protocol, please refer to Meng et al. (2020).
Topics & Concepts
Caenorhabditis elegansCell biologyEpidermis (zoology)BiologyIn vivoGeneticsAnatomyGeneGenetics, Aging, and Longevity in Model OrganismsBacterial Genetics and BiotechnologyPhotosynthetic Processes and Mechanisms