PTENP1 is a ceRNA for PTEN: it’s CRISPR clear
Marianna Vitiello, Monica Evangelista, Yang Zhang, Leonardo Salmena, Pier Paolo Pandolfi, Laura Poliseno
Abstract
Here we apply state-of-the-art CRISPR technologies to study the impact that PTENP1 pseudogene transcript has on the expression levels of its parental gene PTEN, and hence on the output of AKT signaling in cancer. Our data expand the repertoire of approaches that can be used to dissect competing endogenous RNA (ceRNA)-based interactions, while providing further experimental evidence in support of the very first one that we discovered.
Topics & Concepts
Competing endogenous RNAPTENCRISPRPseudogeneBiologyComputational biologymicroRNAGeneGeneticsRNABioinformaticsPI3K/AKT/mTOR pathwayCancer researchLong non-coding RNAGenomeSignal transductionCancer-related molecular mechanisms researchPI3K/AKT/mTOR signaling in cancerCRISPR and Genetic Engineering