Resolving the metabolism of monolignols and other lignin-related aromatic compounds in Xanthomonas citri
Damaris Batistão Martim, Anna Julyana Viana Chianca Brilhante, Augusto R. Lima, Maria Paula Paixão, Joaquim Martins, Fernanda Miyuki Kashiwagi, Lúcia D. Wolf, Mariany S Costa, Fabrícia Farias de Menezes, Rafaela Prata, Matheus Coutinho Gazolla, Juliana A. Aricetti, Gabriela Félix Persinoti, George J. M. Rocha, P.O. Giuseppe
Abstract
Lignin, a major plant cell wall component, has an important role in plant-defense mechanisms against pathogens and is a promising renewable carbon source to produce bio-based chemicals. However, our understanding of microbial metabolism is incomplete regarding certain lignin-related compounds like p-coumaryl and sinapyl alcohols. Here, we reveal peripheral pathways for the catabolism of the three main lignin precursors (p-coumaryl, coniferyl, and sinapyl alcohols) in the plant pathogen Xanthomonas citri. Our study demonstrates all the necessary enzymatic steps for funneling these monolignols into the tricarboxylic acid cycle, concurrently uncovering aryl aldehyde reductases that likely protect the pathogen from aldehydes toxicity. It also shows that lignin-related aromatic compounds activate transcriptional responses related to chemotaxis and flagellar-dependent motility, which might play an important role during plant infection. Together our findings provide foundational knowledge to support biotechnological advances for both plant diseases treatments and conversion of lignin-derived compounds into bio-based chemicals.