Gefitinib conjugated PEG passivated graphene quantum dots incorporated PLA microspheres for targeted anticancer drug delivery
Abhishek Gautam, Kaushik Pal
Abstract
In the present study, polyethylene Glycol passivated Graphene Quantum Dots (PEG-GQDs) were successfully synthesized via the hydrothermal method. Furthermore, for the synthesis of anticancer drug loaded GQD embedded microspheres, the anticancer drug was mixed with synthesized PEG-GQD. As prepared, Gefitinib-PEG-GQDs were incorporated into poly-lactic acid (PLA) microspheres using poly-vinyl-acetate (PVA) as surfactant via solvent evaporation technique and single emulsification method. The successful synthesis of anticancer drug loaded microspheres was confirmed by several characterization techniques, including Field-Emission Scanning Electron Microscopy (FE-SEM), which shows the morphology of microspheres, Fourier Transform Infrared Spectroscopy (FTIR) analysis gives an idea about functional group present in the microspheres. X-ray diffraction (XRD) provides information about the crystallinity of the samples respectively. The drug release characteristics were determined by UV-Vis spectrophotometric analysis. Moreover, the in-vitro cell-based cytotoxicity assay indicated almost insignificant cytotoxicity of the NCI–H522 cell line (Human, Lung, Non-small cell lung cancer).