Eco-friendly synthesis of MnO2 nanoparticles using Saraca asoca leaf extract and evaluation of in vitro anticancer activity
Sanjay S. Majani, Sreelakshmi Sathyan, Megha Vadakkethil Manoj, N.A. Vinod, Sushma Pradeep, Chandan Shivamallu, Venkatachalaiah K.N, Shiva Prasad Kollur
Abstract
Manganese oxide nanoparticles (MnO2 NPs) were synthesized through a simple one-pot green synthesis method using Saraca asoca leaves extract (SA-MnO2 NPs), and their physicochemical properties were characterized using various analytical techniques, including electron microscopy (FESEM and TEM), X-ray diffraction (XRD), and electron diffracted X-ray spectroscopy (EDAX). The as-synthesized MnO2 NPs had a highly crystalline structure with a calculated crystalline size of approximately 18 nm and a d-spacing value of 0.216 nm. The morphology of the SA-MnO2 NPs was like stacked cubes with high elemental purity, as confirmed by the EDAX spectrum. Further, confirming the as-obtained crystalline size and d-spacing value from the XRD analysis, the samples were subjected to anti-cancer activities to evaluate their reactance against cancer cell lines. The in vitro anti-cancer activity of the synthesized material was assessed against two breast cancer cell lines, namely MCF7 and MDA-MB-231. The results showed that SA-MnO2 NPs exhibited significantly lower levels of cytotoxicity against these cell lines, indicating their potential as an effective anticancer agent. Further, the IC50 value of SA-MnO2 NPs at 24 h was greater than 20 μg/mL. Therefore, the synthesized SA-MnO2 NPs could be a promising candidate for developing novel breast cancer treatment therapies.