Prediction and Utilization of Malondialdehyde in Exotic Pine Under Drought Stress Using Near-Infrared Spectroscopy
Yini Zhang, Qifu Luan, Jingmin Jiang, Yanjie Li
Abstract
Drought is a major abiotic stress that adversely affects the growth and productivity of plants. Malondialdehyde (MDA), a substance produced by membrane lipids in response to reactive oxygen species (ROS), can be used as a drought indicator to evaluate the degree of plasma membrane damage and the ability of plants to drought stress tolerance. Still measuring MDA is usually a labor- and time-consuming task. In this study, near-infrared (NIR) spectroscopy combined with partial least squares (PLS) was used to obtain rapid and high-throughput measurements of MDA, and the application of this technique to plant drought stress experiments was also investigated. Two exotic conifer tree species, namely, slash pine ( Pinus elliottii ) and loblolly pine ( Pinus taeda ), were used as plant material exposed to drought stress; different types of spectral preprocessing methods and important feature-selection algorithms were applied to the PLS model to calibrate it and obtain the best MDA-predicting model. The results show that the best PLS model is established via the combined treatment of detrended variable–significant multivariate correlation algorithm (DET-sMC), where latent variables (LVs) were 6. This model has a respectable predictive capability, with a correlation coefficient ( R 2 ) of 0.66, a root mean square error (RMSE) of 2.28%, and a residual prediction deviation (RPD) of 1.51, and it was successfully implemented in drought stress experiments as a reliable and non-destructive method to detect the MDA content in real time.