Spatiotemporal Resolution of Conformational Changes in Biomolecules by Combining Pulsed Electron–Electron Double Resonance Spectroscopy with Microsecond Freeze-Hyperquenching
Tobias Hett, Tobias Zbik, Shatanik Mukherjee, Hideto Matsuoka, Wolfgang Bönigk, Daniel Klose, Christophe Rouillon, Norbert Brenner, Sebastian Peuker, Reinhard Klement, Heinz‐Jürgen Steinhoff, Helmut Grubmüller, Reinhard Seifert, Olav Schiemann, U. Benjamin Kaupp
Abstract
potassium channel occurs within about 150 μs and can be resolved with angstrom precision. Thus, this approach holds great promise for obtaining 4D landscapes of conformational changes in biomolecules.
Topics & Concepts
MicrosecondChemistryBiomoleculeResolution (logic)AngstromSpectroscopyElectron crystallographyResonance (particle physics)Chemical physicsCrystallographyAtomic physicsElectron diffractionOpticsPhysicsDiffractionBiochemistryArtificial intelligenceQuantum mechanicsComputer scienceElectron Spin Resonance StudiesSpectroscopy and Quantum Chemical StudiesAdvanced NMR Techniques and Applications