PenA, a penicillin-binding protein-type thioesterase specialized for small peptide cyclization
Kenichi Matsuda, Kei Fujita, Toshiyuki Wakimoto
Abstract
Penicillin-binding protein-type thioesterases (PBP-type TEs) are a recently identified group of peptide cyclases that catalyze head-to-tail macrolactamization of nonribosomal peptides. PenA, a new member of this group, is involved in the biosyntheses of cyclic pentapeptides. In this study, we demonstrated the enzymatic activity of PenA in vitro, and analyzed its substrate scope with a series of synthetic substrates. A comparison of the reaction profiles between PenA and SurE, a representative PBP-type TE, showed that PenA is more specialized for small peptide cyclization. A computational model provided a possible structural rationale for the altered specificity for substrate chain lengths.
Topics & Concepts
ThioesterasePeptideCyclic peptideBiochemistryNonribosomal peptidePenicillin binding proteinsChemistryStereochemistrySubstrate (aquarium)EnzymePenicillinBiosynthesisBiologyAntibioticsEcologyBiochemical and Structural CharacterizationMicrobial Natural Products and BiosynthesisChemical Synthesis and Analysis