CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice
Chih‐Hao Wang, Morten Lundh, Accalia Fu, Rókus Kriszt, Tian Lian Huang, Matthew D. Lynes, Luiz Osório Leiria, Farnaz Shamsi, Justin Darcy, Bennett Greenwood, Niven R. Narain, Vladimir Tolstikov, Kyle Smith, Brice Emanuelli, Young‐Tae Chang, Susan J. Hagen, Nika N. Danial, Michael A. Kiebish, Yu‐Hua Tseng
Abstract
-nitrosothiols and nitrite in red blood cells to activate endogenous brown fat and improved glucose homeostasis in recipient animals. Together, these data demonstrate the utility of using CRISPR-Cas9 technology to engineer human white adipocytes to display brown fat-like phenotypes and may open up cell-based therapeutic opportunities to combat obesity and diabetes.
Topics & Concepts
CRISPRObesityMetabolic syndromeMedicineBiologyEndocrinologyBioinformaticsInternal medicineGeneGeneticsAdipose Tissue and MetabolismAdipokines, Inflammation, and Metabolic DiseasesSirtuins and Resveratrol in Medicine