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Microbially-Induced-Calcite-Precipitation (MICP): A biotechnological approach to enhance the durability of concrete using Bacillus pasteurii and Bacillus sphaericus

Amal A. Nasser, Noha M. Sorour, Mohamed A. Saafan, Rateb Abbas

2022Heliyon110 citationsDOIOpen Access PDF

Abstract

(BS) were added into mortar mixtures with 0.25% and 0.5% cement weight. All treated samples exhibited a significant decline in water uptake, capillary permeability, and volume of permeable voids, as compared to control with no bacteria. All treated samples showed significant increase in compressive strength by 28-50%, after 28 days of curing. At the age of 120 days, the flexural strength of all treated samples was significantly increased by 19.29-65.94%. SEM imaging and EDAX confirmed that treated samples were denser with less voids due to MICP. DTA verified that the calcite amount and the crystallinity degree were improved in treated samples. Load deflection of bacterial Reinforced-Laminates had less deformation than control. Reloaded bacterial Reinforced-Laminates exhibited excellent restoration of physico-mechanical properties and performance, after 28, 90, and 120 days, confirming the healing process. Microbial self-healing is an innovative approach for continuous repair of micro-cracks in concrete, improving its durability, thus can reduce the maintenance costs.

Topics & Concepts

DurabilityMaterials scienceFlexural strengthCrystallinityComposite materialCompressive strengthWater retentionCuring (chemistry)CalciteMortarCementBacillus sphaericusChemistryBacillalesMineralogySoil waterBiologyEcologyBacteriaBacillus subtilisGeneticsMicrobial Applications in Construction MaterialsCorrosion Behavior and InhibitionMarine Sponges and Natural Products
Microbially-Induced-Calcite-Precipitation (MICP): A biotechnological approach to enhance the durability of concrete using Bacillus pasteurii and Bacillus sphaericus | Litcius