Litcius/Paper detail

Re-evaluating what makes a phage unsuitable for therapy

Bradley W. M. Cook, Alexander P. Hynes

2025npj Antimicrobials and Resistance39 citationsDOIOpen Access PDF

Abstract

One of the biggest challenges in phage therapy is selecting the 'right' phage. What constitutes a 'good' phage is unclear, but regulators are converging on exclusion criteria for 'bad' phages. Here, we examine three commonly applied criteria: the lack of virulence/antibiotic resistance, the inability to transduce, and being strictly lytic (virulent). We assess the risk posed, the tools for determining the criteria, and the potential impact of the criteria.

Topics & Concepts

Lytic cycleVirulencePhage therapyBacteriophageAntibiotic resistanceVirologyBiologyMicrobiologyAntibioticsGeneticsVirusEscherichia coliGeneBacteriophages and microbial interactionsPlant Virus Research StudiesMicrobial infections and disease research