Automatic Calculation of the Kinetic Parameters of Enzymatic Reactions with Their Standard Errors Using Microsoft Excel
Motomitsu Kitaoka
Abstract
We created a Microsoft Excel file, Enzyme_Kinetics_Calculator, which includes macro programs that automatically calculates kinetic parameters for typical kinetic equations of enzymatic reactions, accompanied by their standard errors, by minimizing the residual sum of squares thereof. The [S]-v plot is automatically drawn with the theoretical lines and, similarly, the 1/[S]-1/v plot in the case of linear theoretical lines. Enzyme_Kinetics_Calculator is available as a supplementary file for this paper (see J. Appl. Glycosci. Web site).
Topics & Concepts
Microsoft excelCalculatorMacroComputer sciencePlot (graphics)Kinetic energyResidualCurve fittingKineticsAlgorithmThermodynamicsChemistryMathematicsStatisticsProgramming languageOperating systemPhysicsQuantum mechanicsEnzyme function and inhibitionEnzyme Catalysis and ImmobilizationElectrochemical sensors and biosensors