Cellvibrio polysaccharolyticus sp. nov., a cellulolytic bacterium isolated from agricultural soil
Ákos Tóth, Rózsa Máté, József Kutasi, Ildikó Bata‐Vidács, Erika Tóth, András Táncsics, Gábor M. Kovács, István Nagy, József Kukolya
Abstract
A novel Gram-reaction-negative bacterial strain, designated Ka43 T , was isolated from agricultural soil and characterised using a polyphasic approach to determine its taxonomic position. On the basis of 16S rRNA gene sequence analysis, the strain shows highest similarity (97.1 %) to Cellvibrio diazotrophicus E50 T . Cells of strain Ka43 T are aerobic, motile, short rods. The major fatty acids are summed feature 3 (C 16 : 1 ω 7 c and/or iso-C 15 : 0 2-OH), C 18 : 1 ω 7 c and C 16 : 0 . The only isoprenoid quinone is Q-8. The polar lipid profile includes phosphatidylethanolamine, phosphatidylglycerol, four phospholipids, two lipids and an aminolipid. The assembled genome of strain Ka43 T has a total length of 4.2 Mb and the DNA G+C content is 51.6 mol%. Based on phenotypic data, including chemotaxonomic characteristics and analysis of the 16S rRNA gene sequences, it was concluded that strain Ka43 T represents a novel species in the genus Cellvibrio , for which the name Cellvibrio polysaccharolyticus sp. nov. is proposed. The type strain of the species is strain Ka43 T (=LMG 31577 T =NCAIM B.02637 T ).