Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry
Nils Helge Schebb, Nadja Kampschulte, Gerhard Hagn, Kathrin Plitzko, Sven W. Meckelmann, Soumita Ghosh, Robin Joshi, Julia Kuligowski, Dajana Vuckovic, Marina Tonetti Botana, Ángel Sánchez‐Illana, Fereshteh Zandkarimi, Aditi Das, Jun Yang, Louis Schmidt, Antonio Checa, Helen M. Roche, Aaron M. Armando, Matthew L. Edin, Fred B. Lih, Juan J. Aristizabal‐Henao, Sayuri Miyamoto, Francesca Giuffrida, Arieh Moussaieff, Rosário Domingues, Michael Rothe, Christine Hinz, Ujjalkumar Subhash Das, Katharina M. Rund, Ameer Y. Taha, R. Hofstetter, Markus Werner, Oliver Werz, Astrid S. Kahnt, Justine Bertrand‐Michel, Pauline Le Faouder, Robert Gurke, Dominique Thomas, Federico Torta, Ivana Milic, Irundika H.K. Dias, Corinne M. Spickett, Denise Biagini, Tommaso Lomonaco, Helena Idborg, Jun‐Yan Liu, Maria Fedorova, David A. Ford, Anne Barden, Trevor A. Mori, Paul D. Kennedy, Kirk M. Maxey, Julijana Ivanišević, Héctor Gallart‐Ayala, Cécile Gladine, Markus R. Wenk, Jean-Marie Galano, Thierry Durand, Ken D. Stark, Coral Barbas, Ulrike Garscha, Stacy G. Wendell, Uta Ceglarek, Nicolas Flamand, Julian L. Griffin, Robert Ahrends, Makoto Arita, Darryl C. Zeldin, Francisco J. Schöpfer, Oswald Quehenberger, Randall K. Julian, Anna Nicolaou, Ian A. Blair, Michael P. Murphy, Bruce D. Hammock, Bruce Α. Freeman, Gerhard Liebisch, Charles N. Serhan, Harald Köfeler, P.‐J. Jakobsson, Dieter Steinhilber, Michael H. Gelb, Michal Holčapek, Ruth Andrew, Martin Giera, Garret A. FitzGerald, Robert C. Murphy, John W. Newman, Edward A. Dennis, Kim Ekroos, Ginger L. Milne, Miguel A. Gijón, Hubert W. Vesper, Craig E. Wheelock, Valerie B. O’Donnell
Abstract
Several oxylipins are potent lipid mediators that regulate diverse aspects of health and disease and whose quantitative analysis by liquid chromatography-mass spectrometry (LC-MS) presents substantial technical challenges. As members of the lipidomics community, we developed technical recommendations to ensure best practices when quantifying oxylipins by LC-MS.