Generation of Multivalent Nanobody-Based Proteins with Improved Neutralization of Long α-Neurotoxins from Elapid Snakes
Jack Wade, Charlotte Rimbault, Hanif Ali, Line Ledsgaard, Esperanza Rivera‐de‐Torre, Maher Abou Hachem, Kim Boddum, Nadia Mirza, Markus‐Frederik Bohn, Siri Aastedatter Sakya, Fulgencio Ruso‐Julve, Jan Terje Andersen, Andreas H. Laustsen
Abstract
. This engineering approach offers a means of altering the valence, endosomal recyclability, and hydrodynamic size of existing nanobody-based therapeutics in a simple plug-and-play fashion and can thus serve as a technology for researchers tailoring therapeutic properties for improved neutralization of soluble targets such as snake toxins.
Topics & Concepts
ChemistryNeutralizationAntibodyImmunologyBiologyVenomous Animal Envenomation and StudiesBiochemical and Structural CharacterizationForce Microscopy Techniques and Applications