Erratum To: Developmental function and state transitions of a gene expression oscillator in Caenorhabditis elegans
Milou W.M. Meeuse, Yannick P. Hauser, Lucas J Morales Moya, Gert‐Jan Hendriks, Jan Eglinger, Guy Bogaarts, Charisios D. Tsiairis, Helge Großhans
Abstract
Since the publication of the above manuscript, the authors have noticed that the experimental temperature given for a replicate experiment in the Materials and Methods section (subsection “RNA sequencing”) is incorrect. The updated description of RNA sequencing experiment is provided below with the correction indicated in bold. The error does not affect any interpretation or conclusion of the original paper. The authors apologize for this error and any inconvenience it might have caused. For RNA sequencing, synchronized L1 worms, obtained by hatching eggs in the absence of food, were cultured at 25°C and collected hourly from 1 h until 15 h of larval development, or 5 h until 48 h of larval development, for L1–L2 time course (TC1) and L1–YA time course (TC2), respectively. A replicate experiment was performed from 1 h until 24 h at 25°C (TC4). RNA was extracted in Tri Reagent and DNase-treated as described previously (Hendriks et al, 2014). For TC2 and TC4, libraries were prepared using the TruSeq Illumina mRNA-seq (stranded—high input), followed by the Hiseq50 Cycle Single-end reads protocol on HiSeq2500. For TC1, libraries were prepared using the Illumina TruSeq mRNA-Seq Sample Prep Kit (Strand-sequenced: any), followed by the Hiseq50 Cycle Single-end reads protocol on a HiSeq2500.