Serum metabonomics revealed the mechanism of Ganoderma amboinense polysaccharides in preventing non-alcoholic fatty liver disease (NAFLD) induced by high-fat diet
Fei Ren, Qianping Chen, Meng Chen, Haiming Chen, Yongjie Zhou, Hao Zhang, Weijun Chen
Abstract
Non-alcoholic fatty liver disease (NAFLD) is one of the most widespread chronic diseases. Previous reports showed that Ganoderma amboinense polysaccharide (GAP) could protect the liver. In this study, high-fat diet (HFD) mice were given GAP for 8 weeks to evaluate the feasibility of GAP to prevent NAFLD and explore its mechanism of action. The results showed that GAP effectively prevented the development of NAFLD, at the same time, reduced the body weight, liver weight and blood lipid levels. Through the serum biochemical analysis and metabolomics analysis, it was found that GAP promoted fat transport in the liver by regulating the phosphatidylcholine content in the blood. At the same time, GAP regulated some metabolic pathways and protected the mitochondrial function of HFD-mouse liver cells, which is the basis for the rapid catabolism of lipids. These results indicated that GAP could be used as a potential preventive and therapeutic agent for NAFLD.