Expression of lactate dehydrogenase A and B isoforms in the mouse kidney
Gunars Osis, Amie Traylor, Laurence M. Black, Daryll Spangler, James F. George, Abolfazl Zarjou, Jill W. Verlander, Anupam Agarwal
Abstract
Cellular release of lactate dehydrogenase (LDH) is being used as an injury marker; however, the exact localization of LDH within the nephron remains unclear. We show that LDH isoform A is expressed proximally, whereas isoform B is expressed distally. Both subunit expressions were significantly altered in models of acute kidney injury and chronic kidney disease. Our study provides new insights into basal and postinjury renal lactate metabolism.
Topics & Concepts
Lactate dehydrogenaseGene isoformKidneyBasal (medicine)NephronAcute kidney injuryKidney diseaseBiologyInternal medicineEndocrinologyProtein subunitMedicineBiochemistryEnzymeGeneInsulinAcute Kidney Injury ResearchElectrolyte and hormonal disordersMetabolism and Genetic Disorders