Decoupling Protein Production from Cell Growth Enhances the Site-Specific Incorporation of Noncanonical Amino Acids in <i>E. coli</i>
Meritxell Galindo Casas, Patrick Stargardt, Juergen Mairhofer, Birgit Wiltschi
Abstract
strain BL21(DE3). The target proteins were expressed at high levels, and the ncAA was efficiently incorporated with excellent fidelity while the protein function was preserved.
Topics & Concepts
Protein biosynthesisProtein engineeringAmino acidMutantSynthetic biologyTransfer RNARecombinant DNABiochemistryEscherichia coliStop codonBiologyGreen fluorescent proteinChemistryCell biologyGeneticsRNAGeneEnzymeRNA and protein synthesis mechanismsMonoclonal and Polyclonal Antibodies ResearchBacteriophages and microbial interactions