Unlocking the PIP-box: A peptide library reveals interactions that drive high-affinity binding to human PCNA
Aimee J. Horsfall, Bethiney C. Vandborg, Wioleta Kowalczyk, Theresa Chav, Denis B. Scanlon, Andrew D. Abell, John B. Bruning
Abstract
value of 1.12 nM, from a peptide with PIP-box QTRITEYF. This work showcases the sequence-specific nuances within the PIP-box that are responsible for high-affinity hPCNA binding, which underpins our understanding of how nature tunes hPCNA affinity to regulate DNA replication and repair processes. In addition, these insights will be useful to future design of hPCNA inhibitors.
Topics & Concepts
Surface plasmon resonancePeptideRational designPeptide libraryProliferating cell nuclear antigenDNA replicationDNABinding siteBiophysicsBiologyBiochemistryChemistryComputational biologyPeptide sequenceNanotechnologyGeneticsGeneMaterials scienceNanoparticleDNA Repair MechanismsEnzyme Structure and FunctionProtein Structure and Dynamics