Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down proteomics
Guijie Zhu, Seyed Amirhossein Sadeghi, Morteza Mahmoudi, Liangliang Sun
Abstract
The nanoparticle (NP) protein corona significantly influences the outcome of nanomedicine. We present the first example of top-down proteomics (TDP) measurement of the protein corona using capillary isoelectric focusing-mass spectrometry, identifying seventy proteoforms of 16 cancer-related genes. This technique has the potential to revolutionize our understanding of the protein corona and advance nanomedicine.
Topics & Concepts
NanomedicineProteomicsMass spectrometryIsoelectric focusingNanoparticleChemistryCorona (planetary geology)Corona dischargeNanotechnologyChromatographyMaterials sciencePhysicsBiochemistryGeneEnzymeAstrobiologyVenusElectrodePhysical chemistryAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsAdvanced biosensing and bioanalysis techniques