CD4+ T-Cell Epitope Prediction by Combined Analysis of Antigen Conformational Flexibility and Peptide-MHCII Binding Affinity
Tysheena P. Charles, Daniel L. Moss, Pawan Bhat, Peyton W. Moore, Nicholas Kummer, Avik Bhattacharya, Samuel J. Landry, Ramgopal R. Mettu
Abstract
-MHCII-peptide affinity reached 36%. When MHCII allele specificity was also diverse, such as in human immunity, prediction of dominant epitopes by APL alone reached 42% when using a stringent scoring threshold. Because dominant CD4+ epitopes tend to occur in conformationally stable antigen domains, crystal structures typically are available for analysis by APL, and thus, the requirement for a crystal structure is not a severe limitation.
Topics & Concepts
EpitopeLinear epitopeAntigenConformational epitopeProteolysisEpitope mappingBiologyChemistryPeptideMolecular biologyBiochemistryEnzymeImmunologyvaccines and immunoinformatics approachesImmunotherapy and Immune ResponsesT-cell and B-cell Immunology