Ribosome-Mediated Attenuation of <i>vga</i> (A) Expression Is Shaped by the Antibiotic Resistance Specificity of Vga(A) Protein Variants
Vladimir Vimberg, Jorunn Pauline Cavanagh, Michaela Novotná, Jakub Lenart, Bich Nguyen Thi Ngoc, Jana Vesela, Maria Pain, Markéta Koběrská, Gabriela Balíková Novotná
Abstract
Vga(A) protein variants confer different levels of resistance to lincosamides, streptogramin A, and pleuromutilins (LS A P) by displacing antibiotics from the ribosome. Here, we show that expression of vga (A) variants from Staphylococcus haemolyticus is regulated by cis -regulatory RNA in response to the LS A P antibiotics by the mechanism of ribosome-mediated attenuation. The specificity of induction depends on Vga(A)-mediated resistance rather than on the sequence of the riboregulator.
Topics & Concepts
Video Graphics ArrayAntibiotic resistanceBiologyRibosomeAntibioticsGeneticsMicrobiologyGeneRNAComputer scienceChipTelecommunicationsImmune Response and InflammationRNA and protein synthesis mechanismsBacterial Genetics and Biotechnology