Nek8445, a protein kinase required for microtubule regulation and cytokinesis in <i>Giardia lamblia</i>
Kelly M. Hennessey, Germain C. M. Alas, Ilse Rogiers, Renyu Li, E.A. Merritt, Alexander R. Paredez
Abstract
Here we study the role of Nek8445 in regulating cell division and microtubule array organization in Giardia. Depletion of Nek8445 results in 87% of cells being stalled or blocked in cytokinesis. Nek8445 regulates ventral disk organization, funis formation, axoneme exit, and cell shape, all of which contribute to the observed cytokinesis defects.
Topics & Concepts
BiologyCell biologyCytokinesisAxonemeMicrotubuleCell divisionKinaseLive cell imagingNocodazoleCytoskeletonFlagellumCellGeneticsGeneParasitic Infections and DiagnosticsToxoplasma gondii Research StudiesProtist diversity and phylogeny