Protective effect of the newly synthesized and characterized charge transfer (CT) complex against arecoline induced toxicity in third-instar larvae of transgenic <i>Drosophila melanogaster (hsp70-lacZ)Bg</i><sup><i>9</i></sup>: experimental and theoretical mechanistic insights
Sonam Shakya, Ishaat M. Khan, Barkha Shakya, Yasir Hasan Siddique, Himanshi Varshney, Smita Jyoti
Abstract
. The possible mechanism of this effect was explored through fluorescence and UV-vis spectroscopy. The possibility of suppression of arecoline action on the muscarinic acetylcholine receptor 1-G11 protein complex (found in the cell membrane) in the presence of CL was studied theoretically by molecular docking. Density functional theory (DFT) also theoretically supported various aspects of the designed material concerning the energy profile of the orbitals (HOMO-LUMO) as well as the energy minimized structure. Furthermore, time dependent (TD) DFT corroborated the electronic properties of the designed material.
Topics & Concepts
Drosophila melanogasterInstarArecolineLarvaTransgeneBiologyToxicologyMaterials scienceBiochemistryBotanyGeneReceptorMuscarinic acetylcholine receptorFree Radicals and AntioxidantsMetal complexes synthesis and propertiesPhotochemistry and Electron Transfer Studies