Roll-to-Roll Manufacturing of Integrated Immunodetection Sensors
Christina Liedert, Lauri Rannaste, Annukka Kokkonen, Olli‐Heikki Huttunen, Ralph Liedert, Jussi Hiltunen, Leena Hakalahti
Abstract
Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included a plasma separation membrane for simple blood filtration, immobilized antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. These functionalities were designed to be compatible with R2R processing, which was demonstrated using pilot-scale printing lines producing 60 devices in an hour. The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL) fluorescence-based immunodetection of C-reactive protein from 20 μL of whole blood.