Guanidine-based DNA extraction with silica-coated beads or silica spin columns v1
Dominik Buchner
Abstract
This protocol describes how to extract DNA from samples lysed as described in using guanidine hydrochloride and ethanol-based buffer combined with silica-coated magnetic beads or silica spin columns. The spin column protocol can be used either with centrifugation or, alternatively, a vacuum manifold. Compared to approaches with magnetic beads, with silica spin column protocols higher yields are possible since the amount of lysate used can be increased. The bead-based protocol is an automation-friendly alternative.
Topics & Concepts
ChromatographyGuanidineMagnetic beadCentrifugationDNA extractionChemistryHydrochlorideLysisBeadMaterials scienceExtraction (chemistry)Organic chemistryComposite materialBiochemistryGenePolymerase chain reactionChemical Synthesis and AnalysisAnalytical Chemistry and ChromatographyGene expression and cancer classification