Cryo-fluorescence micro-optical sectioning tomography for volumetric imaging of various whole organs with subcellular resolution
Lei Deng, Jianwei Chen, Yafeng Li, Yutong Han, Guoqing Fan, Jie Yang, Dongjian Cao, Bolin Lu, Kefu Ning, Shuo Nie, Zoutao Zhang, Dan Shen, Yunfei Zhang, Wenbin Fu, Wei Eric Wang, Ying Wan, Sha Li, Yu‐Qi Feng, Qingming Luo, Jing Yuan
Abstract
hybridization imaging to verify the compatibility of multi-omics measurements. The results demonstrated that cryo-fMOST is capable of acquiring high-resolution morphological details of various whole organs and may be potentially useful for spatial multi-omics.
Topics & Concepts
MicrotomeOptical sectioningBiomedical engineeringFluorescence-lifetime imaging microscopyMicroscopyTomographyLight sheet fluorescence microscopyMaterials scienceResolution (logic)Fluorescence microscopeFluorescenceVisualizationComputer scienceOpticsArtificial intelligencePathologyMedicinePhysicsAdvanced Fluorescence Microscopy TechniquesSingle-cell and spatial transcriptomicsPhotoacoustic and Ultrasonic Imaging