Middle-out sequence confirmation of CRISPR/Cas9 single guide RNA (sgRNA) using DNA primers and ribonuclease T1 digestion
Steven Chin, Alexandre Goyon, Kelly Zhang, Kenji L. Kurita
Topics & Concepts
RibonucleaseOligonucleotideRNase PSubgenomic mRNAGuide RNACas9ChemistryRNase HRNAPrimer (cosmetics)Ribonuclease T1DNAComputational biologyCRISPRBiologyBiochemistryGeneOrganic chemistryCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery