A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample
Yusuke Okazaki, Tuyen Thi My Nguyen, Arisa Nishihara, Hisashi Endo, Hiroyuki Ogata, Shin‐ichi Nakano, Hideyuki Tamaki
Abstract
We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10-15 kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50 ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications.