Fit-Seq2.0: An Improved Software for High-Throughput Fitness Measurements Using Pooled Competition Assays
Fangfei Li, Jason Tarkington, Gavin Sherlock
Abstract
The fitness of a genotype is defined as its lifetime reproductive success, with fitness itself being a composite trait likely dependent on many underlying phenotypes. Measuring fitness is important for understanding how alteration of different cellular components affects a cell's ability to reproduce. Here, we describe an improved approach, implemented in Python, for estimating fitness in high throughput via pooled competition assays.
Topics & Concepts
BiologyCompetition (biology)ThroughputSoftwareEvolutionary biologyComputational biologyEcologyComputer scienceOperating systemWirelessSingle-cell and spatial transcriptomicsViral Infections and Immunology ResearchBiotin and Related Studies