Host–Guest Allosteric Control of an Artificial Phosphatase
Joanna Czescik, Yanchao Lyu, Samuele Neuberg, Paolo Scrimin, Fabrizio Mancin
Abstract
The activity of many enzymes is regulated by associative processes. To model this mechanism, we report here that the conformation of an unstructured bimetallic Zn(II) complex can be controlled by its inclusion in the cavity of a γ-cyclodextrin. This results in the formation of a catalytic bimetallic site for the hydrolytic cleavage of the RNA model substrate HPNP, whose reactivity is 30-fold larger with respect to the unstructured complex. Competitive inhibition with 1-adamantanecarboxylate displaces the metal complex from the cyclodextrin decreasing the reactivity.
Topics & Concepts
ChemistryBimetallic stripAllosteric regulationReactivity (psychology)CyclodextrinKinetic controlCleavage (geology)EnzymeStereochemistryPhosphataseCatalysisCombinatorial chemistryBiochemistryEngineeringPathologyGeotechnical engineeringMedicineAlternative medicineFracture (geology)Supramolecular Chemistry and ComplexesMolecular Sensors and Ion DetectionSupramolecular Self-Assembly in Materials