Studies on the selectivity of the SARS-CoV-2 papain-like protease reveal the importance of the P2′ proline of the viral polyprotein
H. T. Henry Chan, Lennart Brewitz, Petra Lukacik, Claire Strain‐Damerell, Martin Walsh, Christopher J. Schofield, Fernanda Duarte
Abstract
Computational modeling of how SARS-CoV-2 PL pro binds viral polyprotein-derived oligopeptide substrates reveals that a proline located at the P2′ position promotes catalysis, validated by residue substitutions and mass spectrometry-based analyses.
Topics & Concepts
PapainProteaseSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)VirologyChemistryCoronavirus disease 2019 (COVID-19)ProteasesBiochemistryBiologyEnzymeMedicineInternal medicineInfectious disease (medical specialty)DiseaseSARS-CoV-2 and COVID-19 ResearchViral gastroenteritis research and epidemiologyRNA Interference and Gene Delivery