Direct Quantification of Damaged Nucleotides in Oligonucleotides Using an Aerolysin Single Molecule Interface
Jiajun Wang, Meng‐Yin Li, Jie Yang, Yaqian Wang, Xueyuan Wu, Jin Huang, Yi‐Lun Ying, Yi‐Tao Long
Abstract
G, I) without labeling and quantify modification sites under the mixed heterocomposition conditions of the oligonucleotide. Such a nanopore electrochemistry approach could be further applied to diagnose genetic diseases at high sensitivity.
Topics & Concepts
AerolysinNanoporeOligonucleotideDNANanopore sequencingComputational biologyInosineBiophysicsDNA sequencingNanotechnologyMaterials scienceBiologyGeneticsBiochemistryGeneEnzymeVirulenceNanopore and Nanochannel Transport StudiesFuel Cells and Related MaterialsAdvanced biosensing and bioanalysis techniques