3D printed microfluidic chemiluminescence PoC device with self-powering and integrated incubating system: Validation via ALP detection on disposable µPADs
Pavar Sai Kumar, Srinivasan Madapusi, Sanket Goel
Abstract
Point-of-Care (PoC) devices have become the fundamental requirement for rapid diagnostics and quantitative analysis at locations convenient to patients. This work reports a fully portable, pocket-friendly, self-powering, and temperature-controlled 3D printed chemiluminescence (CL) device. Moreover, the CL device leveraged the omnipresent smartphone as a detector unit to capture the CL signals. The device also contains a removable magnetic locking smartphone holder, making it universal. Further, the CL device integrates a microfluidic µPAD based detection methodology to monitor the abnormal levels of crucial bio-analyte alkaline phosphatase (ALP). The alkaline phosphatase (ALP) hydrolysis reaction with l -Ascorbic acid 2 phosphate releases a by-product, ascorbic acid (AA). The produced AA is a radical scavenger that quenches the CL signal, thereby facilitating the detection of ALP . Excellent performance of the developed CL device was observed in detecting the ALP with good linearity ranging from 1 mU/L to 10 mU/L and a detection limit of 0.59 mU/L. Subsequently, the device was tested for real-time ALP detection in human blood serum samples. Interestingly, the obtained results were less than 7 % error, compared to clinical samples. The developed fully portable 3D printed CL device with integrated electronics can be a future benchmark for mass production of susceptible devices in detecting various analytes.