<i>Bmal1</i> promotes prostaglandin E<sub>2</sub> synthesis by upregulating <i>Ptgs2</i> transcription in response to increasing estradiol levels in <i>day 4</i> pregnant mice
Lijia Zhao, Luda Yang, Jing Zhang, Yaoyao Xiao, Meina Wu, Tiantian Ma, Xiaoyu Wang, Linlin Zhang, Haizhen Jiang, Hsu‐Wen Chao, Aihua Wang, Yaping Jin, Huatao Chen
Abstract
Rhythmic expression of Bmal1 and Ptgs2 was observed in the uterus isolated from D3.5–4.5 of pregnant mice. E 2 increased the expression of Bmal1 and Ptg2 in UESCs isolated from mice on D4. The expression of Ptgs2 was significantly decreased in Bmal1-siRNA treated UESCs. Bmal1 knockdown also inhibited PGE 2 synthesis. Thus, these data suggest that Bmal1 in mice promotes PGE 2 synthesis by upregulating Ptgs2 in response to increases in E 2 on D4 of pregnancy.
Topics & Concepts
Gene knockdownInternal medicineEndocrinologyProstaglandin fUterusProstaglandinChemistryPregnancyTranscription (linguistics)Prostaglandin E2AndrologyBiologyMedicineGeneBiochemistryGeneticsPhilosophyLinguisticsEstrogen and related hormone effectsCircadian rhythm and melatoninReproductive Physiology in Livestock