Physiology-forward identification of bile acid–sensitive vomeronasal receptors
Wen Mai Wong, Jie Cao, Xingjian Zhang, Wayne I. Doyle, Luis Leon‐Mercado, Laurent Gautron, Julian P. Meeks
Abstract
imaging. These experiments revealed multiple populations of BA-receptive VSNs with submicromolar sensitivities. We then developed a new physiology-forward approach for identifying AOS ligand-receptor interactions, which we call Fluorescence Live Imaging for Cell Capture and RNA sequencing, or FLICCR-seq. FLICCR-seq analysis revealed five specific V1R family receptors enriched in BA-sensitive VSNs. These studies introduce a powerful new approach for ligand-receptor matching and reveal biological mechanisms underlying mammalian BA chemosensation.
Topics & Concepts
Vomeronasal organIdentification (biology)ReceptorBile acidComputational biologyPhysiologyBiologyBiochemistryEcologyOlfactory and Sensory Function StudiesBiochemical Analysis and Sensing TechniquesAdvanced Chemical Sensor Technologies