Nisin M: a Bioengineered Nisin A Variant That Retains Full Induction Capacity but Has Significantly Reduced Antimicrobial Activity
Michelle O’ Connor, Des Field, Aoife Grainger, Paula M. O’ Connor, Lorraine A. Draper, R. Paul Ross, Colin Hill
Abstract
This study describes the generation of a nisin variant with superior characteristics for use in the NICE protein expression system. The variant, termed nisin M, retains an induction capacity comparable to that of wild-type nisin A but exhibits significantly reduced antimicrobial activity and can therefore be used at concentrations that are normally toxic to the expression host.
Topics & Concepts
NisinAntimicrobialLantibioticsMicrobiologyProtein expressionBiologyChemistryBiochemistryGeneBacteriophages and microbial interactionsProbiotics and Fermented FoodsTransgenic Plants and Applications