One step carboxyl group isotopic labeling for quantitative analysis of intact <i>N</i>-glycopeptides by mass spectrometry
Zhenyu Sun, Guanghui Ji, Guoli Wang, Lei Wei, Ying Zhang, Haojie Lu
Abstract
Here, we have developed an approach termed methylamine stable isotope labeling (MeSIL) to relatively quantify N-glycopeptides through one step labeling. It is the first time that this approach is applied to measure N-glycopeptide changes in huh7 cells after Zika virus infection and it has been revealed that differentially expressed N-glycopeptides played important roles in virus infection at the glycosylation site-specific level.
Topics & Concepts
ChemistryGlycopeptideMass spectrometryGroup (periodic table)ChromatographyIsotopic labelingIsobaric labelingTandem mass spectrometryProtein mass spectrometryOrganic chemistryBiochemistryAntibioticsGlycosylation and Glycoproteins ResearchAdvanced Proteomics Techniques and ApplicationsCarbohydrate Chemistry and Synthesis