Developments and Opportunities for 3D Bioprinted Organoids
Ren Ya, Xue Yang, Zhengjiang Ma, Xin Sun, Yuxin Zhang, Wentao Li, Han Yang, Lei Qiang, Zezheng Yang, Yihao Liu, Changxu Deng, Liang Zhou, Tianchang Wang, Jingsheng Lin, Tao Li, Tao Wu, Jinwu Wang
Abstract
Organoids developed from pluripotent stem cells or adult stem cells are three-dimensional cell cultures possessing certain key characteristics of their organ counterparts, and they can mimic certain biological developmental processes of organs in vitro. Therefore, they have promising applications in drug screening, disease modeling, and regenerative repair of tissues and organs. However, the construction of organoids currently faces numerous challenges, such as breakthroughs in scale size, vascularization, better reproducibility, and precise architecture in time and space. Recently, the application of bioprinting has accelerated the process of organoid construction. In this review, we present current bioprinting techniques and the application of bioinks and summarize examples of successful organoid bioprinting. In the future, a multidisciplinary combination of developmental biology, disease pathology, cell biology, and materials science will aid in overcoming the obstacles pertaining to the bioprinting of organoids. The combination of bioprinting and organoids with a focus on structure and function can facilitate further development of real organs.