Stomatal Regulation and Osmotic Adjustment in Sorghum in Response to Salinity
Pablo Rugero Magalhães Dourado, Edivan Rodrigues de Souza, Monaliza Alves dos Santos, Cíntia Maria Teixeira Lins, Danilo Rodrigues Monteiro, Martha Katharinne Silva Souza Paulino, Bruce Schaffer
Abstract
Sorghum bicolor (L.) Moench, one of the most important dryland cereal crops, is moderately tolerant of soil salinity, a rapidly increasing agricultural problem due to inappropriate irrigation management and salt water intrusion into crop lands as a result of climate change. The mechanisms for sorghum’s tolerance of high soil salinity have not been elucidated. This study tested whether sorghum plants adapt to salinity stress via stomatal regulation or osmotic adjustment. Sorghum plants were treated with one of seven concentrations of NaCl (0, 20, 40, 60, 80, or 100 mM). Leaf gas exchange (net CO2 assimilation (A), transpiration (Tr); stomatal conductance of water vapor (gs), intrinsic water use efficiency (WUE)), and water (Ψw), osmotic (Ψo), and turgor Ψt potentials were evaluated at 40 days after the imposition of salinity treatments. Plants exhibited decreased A, gs, and Tr with increasing salinity, whereas WUE was not affected by NaCl treatment. Additionally, plants exhibited osmotic adjustment to increasing salinity. Thus, sorghum appears to adapt to high soil salinity via both osmotic adjustment and stomatal regulation.