<i>In Situ</i> Live Imaging of Gut Microbiota
Zhi Zhang, Duo Xu, Jianyang Fang, Dai Wang, Jie Zeng, Xiaodong Liu, Shouqiang Hong, Yunxin Xue, Xianzhong Zhang, Xilin Zhao
Abstract
F]fluoro-d-sorbitol as a gut microbiota-specific radioactive tracer and developed a novel PET-CT scan-based imaging method that enables noninvasive, real-time imaging of the overall gut bacterial landscape. The method showed increased spatial resolution when hamsters replaced mice, suggesting that even higher spatial resolution could be achieved with larger animals such as humans. This novel technology establishes the feasibility of investigating spatial-temporal distribution dynamics of gut microbiota with many human diseases.
Topics & Concepts
Gut floraDysbiosisBiologyGastrointestinal tractPreclinical imagingGut–brain axisBacteriaMicrobiologySegmented filamentous bacteriaProbioticLive cell imagingContext (archaeology)ImmunologyFecesMicrobiomeAntibioticsColonizationMetagenomicsGut microbiota and healthClostridium difficile and Clostridium perfringens researchOrthopedic Infections and Treatments