Chemical Composition and in vitro Evaluationof Cytotoxicity, Antioxidant and AntimicrobialActivities of Essential Oil Extracted from MyristicaFragrans Houtt
Moin ud Din, Abid Ali, Muhammad Yasir, Muhammad Idrees Jilani, Sanila Shoaib, Muhammad Latif, Aftab Ahmad, Saima Naz, Farheen Aslam, Munawar Iqbal, Arif Nazir
Abstract
This paper focuses on the chemical composition, antioxidant, cytotoxicity (brine shrimp lethality and hemolytic assay) and antimicrobial activities of essential oil of mace (Myristica fragrans) oil. We have found 33 compounds as evidenced by GC-MS analysis. The most prominent of these compounds are safrole, -phellandrene, 3-p-menthene and L-terpinen-4-ol. Antioxidant activity of the mace essential oil was examined using the free radical scavenging assay (21.95 g/mL), -carotene in linoleic acid and percent inhibition in linoleic acid (67.9 %) system. Four pathogenic fungi, 4 gram positive and 2 gram negative bacteria were used for antimicrobial activity of oil. Aspergillus niger and Streptococcus mutans proved to be most sensitive with MIC values i.e. 1.01 and 1.72 mg/mL, respectively. The mace