Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy
Lara Williams, Sonja Tischlik, Andreas Scherer, Jörg W. A. Fischer, Malte Drescher
Abstract
Photoexcited triplet states are gaining popularity as spin labels in pulsed electron paramagnetic resonance (EPR) spectroscopy. Here, we demonstrate that the fluorophores Eosin Y, Rose Bengal and Atto Thio12 are suitable markers for distance determination by laser-induced magnetic dipole (LaserIMD) spectroscopy in proteins that lack an intrinsic photoexcitable center.
Topics & Concepts
SpectroscopyDipoleSpin (aerodynamics)Site-directed spin labelingMaterials scienceChemistryChemical physicsNuclear magnetic resonancePhysicsElectron paramagnetic resonanceOrganic chemistryThermodynamicsQuantum mechanicsElectron Spin Resonance StudiesLanthanide and Transition Metal ComplexesPhotochemistry and Electron Transfer Studies