In Vitro and Biological Characterization of Dexamethasone Sodium Phosphate Laden pH-Sensitive and Mucoadhesive Hydroxy Propyl β-Cyclodextrin-g-poly(acrylic acid)/Gelatin Semi-Interpenetrating Networks
Nyla Ajaz, Ikram Ullah Khan, Muhammad Irfan, Syed Haroon Khalid, Sajid Asghar, Yasir Mehmood, Muhammad Asif, Usra, Ghulam Hussain, Yasser Shahzad, Shefaat Ullah Shah, Muhammad Usman Munir
Abstract
The current study reports the fabrication and biological evaluation of hydroxy propyl β-cyclodextrin-g-poly(acrylic acid)/gelatin (HP-β-CD-g-poly(AA)/gelatin) semi-interpenetrating networks (semi-IPN) for colonic delivery of dexamethasone sodium phosphate (DSP). The prepared hydrogels showed pH-dependent swelling and mucoadhesive properties. The mucoadhesive strength of hydrogels increased with an increasing concentration of gelatin. Based on the swelling and mucoadhesive properties, AG-1 was chosen as the optimized formulation (0.33% w/w of gelatin and 16.66% w/w of AA) for further analysis. FTIR revealed the successful development of a polymeric network without any interaction with DSP. SEM images revealed a slightly rough surface after drug loading. Drug distribution at the molecular level was confirmed by XRD. In vitro drug release assay showed pH-dependent release, i.e., a minute amount of DSP was released at a pH of 1.2 while 90.58% was released over 72 h at pH 7.4. The optimized formulation did not show any toxic effects on a rabbit’s vital organs and was also hemocompatible, thus confirming the biocompatible nature of the hydrogel. Conclusively, the prepared semi-IPN hydrogel possessed the necessary features, which can be exploited for the colonic delivery of DSP.