Sturgeon protein-derived peptides exert anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via the MAPK pathway
Ruichang Gao, Wanghui Shu, Yang Shen, Quancai Sun, Fan Bai, Jinlin Wang, Dajing Li, Ying Li, Wengang Jin, Li Yuan
Abstract
Previous studies have demonstrated that polypeptides extracted from fish protein possess potential anti-inflammatory effects, but few studies have reported the underlying anti-inflammatory mechanism of sturgeon peptides. Hence, the goal of this study was to explore the peptide sequences with potential anti-inflammatory activity derived from sturgeon muscle protein in the lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Fourteen novel peptides were identified in major muscle proteins by mass spectrometry, and three synthetic peptides – KIWHHTF, VHYAGTVDY, and HLDDALRGQE - decreased the release of inflammatory mediators and inflammatory cytokines (NO, IL-6, and IL-1β), while increasing SOD activity significantly in the LPS-induced RAW264.7 inflammatory model. Western blotting analysis revealed that these peptides suppressed the phosphorylation levels of MAPKs, indicating that these peptides exerted anti-inflammatory effects by inhibiting the MAPK pathway. The novel peptides potentially have application prospects as functional food ingredients.