RatioCRISPR: A ratiometric biochip based on CRISPR/Cas12a for automated and multiplexed detection of heteroplasmic SNPs in mitochondrial DNA
Xiaolong Wu, Yi Zhao, Chuanghao Guo, Conghui Liu, Qianling Zhang, Yong Chen, Yizhen Liu, Xueji Zhang, Xueji Zhang
Topics & Concepts
HeteroplasmyBiochipMitochondrial DNASingle-nucleotide polymorphismCRISPRBiologyGeneticsMolecular biologySNP genotypingComputational biologySNPMolecular diagnosticsLeber's hereditary optic neuropathyGenotypeGeneCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms